Journal: Frontiers in Physiology
Article Title: Impact of polyethylene terephthalate nanoplastics (PET) on fibroblasts: a study on NIH-3T3 cells
doi: 10.3389/fphys.2025.1580682
Figure Lengend Snippet: Representative image (of n = 3 independent experiments) of a 3T3 fibroblast exposed to PET NPs (50 μg/mL) for 24 h visualized in brightfield (C) and confocal microscopy (D) by a 405 nm laser line of A1 NIKON confocal laser scanning unit coupled with a NIKON Ti microscope. A control cell (A,B) is shown for comparison. Objective used 60X oil immersion. (E) % of cells internalizing PET NPs at different NP concentrations (data are expressed as mean ± SEM of three independent experiment).
Article Snippet: NIH-3T3 cells (concentrated 2 × 10 4 per ml) were seeded in a tissue culture-treated μ-Slide 4 well (Ibidi GmbH, Gräfelfing, Germany) for 24 h and then they were incubated with PET NPs for 24 h. After incubation cells were washed three times to remove NPs and were visualized by a 405 nm laser line of A1 NIKON confocal laser scanning unit using coupled with a NIKON Ti microscope.
Techniques: Confocal Microscopy, Microscopy, Control, Comparison